Introduction: this study aimed to determine and compare the viewpoints of nurses in the field of patient safety culture in the hospital Mostafa Khomeini in Tehran. Methods: This cross-sectional descriptive study in 1394 by using the questionnaire "Hospital Survey on Patient Safety Culture" was performed. The study population consisted of all nurses working in university hospitals control. Data analysis was conducted in two levels of descriptive and inferential statistics. Results: 94 of 120 nurses participated in the study. Dimension scores patient safety culture was related to the department of nurses (61/2), patient safety (39/2), safety Hospital (26/2), communications (21/2), the frequency of reporting of adverse events (14 / 2), and the lowest supervisor department (85/1), respectively. Overall, the mean scores of all dimensions at a low level (less than 5.2 average) have been reported. The strongest correlation was found between the department of nurses and supervisors (p=0.000, r=0.44). Conclusion: Senior management support of patient safety, teamwork approach, measure the effectiveness of changes of patient safety, and personnel dealing respectfully with each were of the strengths of the hospital in the field of patient safety culture. Also according to the results Blame culture, lack of personnel, long working hours, lack of encouraging a culture of patient safety, lack of attention to personnel suggestions, the absence of a culture of prevention, inadequate coordination among hospital departments and units were weaknesses that affecting to reduce patient safety culture dimensions. Such studies can clear the way for improved patient safety culture and seeks to improve the quality of health services.