Evaluation of different parts of tourism will cause the optimized resource allocation of development plans and strategies, thereupon evaluating these parts has become essential in various destinations. Rural tourism consider as one of the main forces of developing and welfare of rural economies. In such term, rural tourism supply chain evaluation is regarded as one of the major strategies for developing the rural area. Tourism supply chain evaluation states that how a tourism product is manufactured and when and how will perform in tourists and which outcome it will cause. Despite the undeniable importance of this issue, there has not been any study devoted to tourism supply chain and investigating its performance. This study will evaluate rural tourism supply chain with Data Envelopment Analysis (DEA) in Mesr village, Iran, in 2015 and will offer solutions in way of improvement and enrichment of its performance. The results indicate that five factors of transportation, visitor information, accommodation, infrastructures and attractions function properly and catering services, Entertainment and shopping facilities have inappropriate functions in Mesr tourism supply chain.