Gastrointestinal stromal tumors (GISTs) are the most frequent mesenchymal neoplasms of the gastrointestinal (GI) tract. Management of GIST patients is now based on the clinicopathological features and associated genetic changes. However, the detailed characteristics of GISTs and molecular genetic features have not yet been described in the Vietnamese population.155 patients with primary GISTs were identified who underwent surgery with primary curative intent between 2011 and 2014 at University Medical Center Ho Chi Minh City, Viet Nam. We evaluated clinicopathological features and immunochemical reactions for p53 and Ki-67 on these patients; additionally, molecular profiles of KIT genotyping were also analyzed in 100 cases.The largest portion of GISTs was classified as high risk (43.2%). Of 155 GISTs cases, 52 (33.5%) cases were positive with Ki-67 and 58 (37.4%) cases were positive with p53. The expression of Ki-67 and p53 was correlated with mitotic rate, tumor size tumor, risk assessment, and tumor stage. Out of 100 GISTs cases, the KIT mutation was found in 68% of which 62 (91,2%) were found in exon 11, 2 (2.9%) cases in exon 9 and 4 (5.8%) cases mutated in exon 17. No patient with a mutation was detected in exon 13 was identified. The KIT mutation did not correlate with any clinicopathological features.Expression of Ki-67 and p53 were associated with high risk tumor. The mutation on exon 11 was the most commonly found, followed by exon 17 and exon 9. The KIT mutation was not correlated with any recognized clinicopathological features.