Objective: To assess the knowledge, attitude and practice of prescription writing among interns in medical college. Materials and Methods: A cross-sectional study was conducted among the interns in a Medical College, after taking approval from the Institutional Ethics Committee. Participants were asked to write prescription for a common ailment in a given prescription blank page. A structured questionnaire was designed based on new Medical Council of India (MCI) prescription writing pattern to analyse the prescription. The data was analysed using descriptive statistics. Results: Among 84 interns, most of the participants had written the drug by generic name (95.2%) and have signed in the prescription sheet (98%). None of them were aware of the latest prescription writing pattern recommended by Medical Council of India (MCI). The writing of generic name in capital letter was not done by 97.6% of interns. Conclusion: Our study concluded that interns were not aware of new Medical Council of India (MCI) prescription writing pattern. Majority of interns have made errors while writing the prescription. Thus there is a need for regular formal education regarding prescription writing, before the start of internship. They can be given training under medical education unit regarding the recent updates of prescription writing.