Ionic liquids, known as non-volatile solvents, have potential for realizing microanalysis with a minute quantity of sample. Here we report the measurement of the Id-Vg characteristics during the enzymatic catalytic reactions and streptavidin-biotin binding in ionic liquids by using the graphene FET sensors we fabricated, and successfully monitored the biological reactions in much smaller amount of solvents. These findings suggest the possibility of ionic liquids for application in bio-microanalysis with high sensitivity.