The fresh dried pollen of grape and seedless grape varieties were used as the research material. Each cultivar was stored at room temperature, 5 C, 0 C, -18 C and -40 C respectively. Sucrose 200g / L + boric acid 50mg / L + agar 8g / L as the nutrient substrate, and the comparative study on the germination and culture of the nuclear breed and the seedless cultivar. The results showed that the pollen viability decreased with the increase of storage time at different temperatures. The pollen viability decreased at -18 C and -40 , and the pollen viability decreased at the same temperature The There was a significant difference in pollen viability between different cultivars at the beginning of storage, and the pollen viability of the kernel breed was higher than that of the seedless grape.