The rhizobacterium AVO110, isolated by enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused by Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudate-containing medium with the goal of identifying molecular mechanisms linked to the interaction of this bacterium with A total of 26 STM mutants out-competed by the parental strain in fungal exudate, but not in rich medium, were selected and named rowth-ttenuated utants (GAM). Twenty-one genes were identified as required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators and genes related to the metabolism of hydrocarbons, amino acids, fatty acids and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase () and a regulator of alginate production () were identified as required for efficient colonization of the avocado rhizosphere. Diseases associated with fungal root invasion cause significant losses on fruit tree production worldwide. The bacterium AVO110 controls avocado white root rot disease caused by using mechanisms involving competition for nutrients and niches. Here, a functional genomics approach was conducted to identify bacterial traits involved in the interaction with this fungal pathogen. Our results contribute to a better understanding of the multitrophic interactions established among bacterial biocontrol agents, the plant rhizosphere and the mycelia of soilborne pathogens.