In this study, we investigated self-weighing frequency (SWF) among adults and whether SWF was associated with health markers. We had 533 US adults complete a questionnaire on SWF and other health markers. Respondents were categorized into 4 quartiles of SWF: "Never," "<1x/week," "1x/week," or ">1x/week." Overall, 60.0% of adults weighed <1x/week and 25% never weighed. More frequent self-weighing was associated with less sedentary time, more vigorous physical activity (PA) and several healthier practices including reading nutrition labels, eating whole grains, drinking less soda, self-monitoring of food, PA and weight change, and consuming lower- or no-calorie beverages and sweeteners. Conversely, there was no difference in BMI, age, stress, education, sleep, screen time, or chronic diseases between SWF quartiles. Whereas SWF was associated with some markers of health including vigorous PA and some healthier dietary habits, SWF was not linked to BMI, stress, sleep, or chronic diseases.