Objectives: The aim of this study was to compare the nipple length of the short nipple pregnant women who used nipple puller or not during antenatal care. Materials and Methods: A randomized controlled trial was conducted. The pregnant women who were classified as low risk pregnancy and the nipple length less than 7 millimeters at the first antenatal visit were randomized into two groups; 125 cases of nipple puller use group and 125 cases of routine follow-up group. The first group, the mothers were taught by nurse and practiced nipple puller use twice a day until delivery. The second group or comparison group, the mothers had routine follow-up during antenatal care. When the mothers delivered, the nipple length was measured again at the first day postpartum and compared with the previous measurement. The demographic data and nipple length between both groups were compared using chi-square test and student t-test.Results: The mean nipple length of the nipple puller use and routine follow-up group was not significantly different at first antenatal visit. In routine follow-up group, the mean right nipple length was 5.3 ± 1.4 millimeters at antenatal care clinic and 5.6 ± 1.4 millimeters at postpartum. The mean left nipple length was 5.0 ± 1.2 millimeters at antenatal care clinic and 5.4 ± 1.2 millimeters at postpartum. In nipple puller use group, the mean right nipple length was 4.7 ± 1.5 millimeters at antenatal care clinic and 9.5 ± 1.7 millimeters at postpartum. The mean left nipple length was 5.1 ± 1.6 millimeters at antenatal care clinic and 9.4 ± 1.7 millimeters at postpartum. The nipple length differences between routine follow-up and nipple puller use groups were statistically significant at postpartum (p<0.001).Conclusion: The nipple puller use during antenatal period could increase nipple length.