Nine naphto-γ-pyrones rubrofusarine B (), TMC 256 A1 (), fansecinones A () and B (), aurasperones A (), B () and F (), dianhydro-aurasperone C () and asperpyrone B () were isolated from the marine-derived fungus KMM 4694. Their structures were established based on spectroscopic methods. The effect of the substances on viability and colony formation of human drug-resistant prostate cancer 22Rv1 cell was evaluated.