Effects of biotic aging on the characteristics of biochar and its water-soluble organic products were determined through a one-year laboratory incubation study. Biochar had a positive influence on microbial population size. Without microbial addition, biochars showed little change, except for an obvious increase in oxygen content from 3.2% to 6.3% after one year. By contrast, the carbon (C) content of the biologically-aged biochars continually decreased throughout the incubation at two humidity levels, suggesting that microbes consumed biochar C or encouraged organic matter solubilization. Fourier Transform Infrared Spectroscopy (FTIR) analysis indicated that all aged biochar surfaces showed increases in oxygen-containing functional groups and TG-DTG analysis showed that biologically-aged biochars were less stable than the corresponding abiotically-aged one. The release of dissolved organic matters from biologically-aged biochar logarithmically increasing with time, corresponded with of the pattern of microbe production, suggesting microbial involvement in solubilizing biochar. Combined three-dimensional excitation-emission matrix (3DEEM) and parallel factor (PARAFAC) analyses revealed that fulvic and humic acid-like components were the main water-soluble products of biologically-aged biochar, and these became increasingly rich in O-containing functional groups, i.e. humified, over time. These results highlight the importance of microbes in chemically transforming biochar and the dissolved products of biochar during aging.