: Each of the techniques used in sport is a complex technique requiring a combination of neuromuscular conduction, motor anticipation, and extremely developed proprioception. This is especially the case in martial arts when we deal with a kick or a blow to a specific target. : The main purpose of this study was to determine the kinematic differences in the tested movement pattern among athletes with different levels of advancement in the conditions of kicking: in the air, at a target (a shield), and in direct contact with a competitor. Comparative analysis was performed among 26 players: 13 advanced (group G1) and 13 beginners (group G2). Kinematic data was recorded using an optical motion capture system. The examination consisted of performing three tests of mae-geri kick in sequences of three kicks in three different conditions (without a target, with a static target, and with an opponent). The examination was performed with the back leg and only the moment of kick was analyzed. : The most significant differences were observed in the movement of head, torso, hip, knee, and ankle segments, especially during a kick at a shield. Based on the conducted analysis, we can assume that karate training changes the strategy of neuromuscular control, promoting improvement of mobility pattern efficiency. : Acquiring this type of knowledge can lead to better results, elimination of errors in training, especially in the initial period of training, and the prevention of possible injuries that occur during exercise or competition.