Knowledge acquisition tools facilitate the creation and maintenance of decision support content, but thus far there is little formal investigation of the requirements and desiderata for such tools. This leads to researchers re-inventing the wheel when building knowledge acquisition tools. This study aims to draw up a requirements list for manual knowledge acquisition tools. A systematic review of the literature combined with the opinion of experts on the research team was used to write the initial requirements list. This requirements list was presented to a group of experts and revised according to their feedback. Embase, MEDLINE, IEEE, and ACM were searched, leading to a total of 1628 records. After screening, 199 records were sought for retrieval and 3 studies were included in the review. The initial requirements list consisted of 24 requirements and 15 sub-requirements, covering multiple aspects of knowledge acquisition tools. The expert consensus sessions resulted in 13 new requirements and 4 new sub-requirements, but no changes to the major categories. The final requirements list contained 37 requirements and 21 sub-requirements, divided over 4 categories. This study has shown that much is still unknown about requirements for KA tools , making it complex for developers to design effective and efficient tools. While our results are preliminary, they might prove to be a valuable starting point for developers and researchers.