Plant functions are governed by complex regulatory mechanisms that operate across diverse cell types in various tissues. However, the challenge of dissecting plant tissues has hindered the widespread application of single-cell technologies in plant research. Recent advancements in single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have propelled the field forward. scRNA-seq enables the examination of gene expression at the single-cell level, while ST preserves the spatial context of cellular organization. While previous reviews have discussed the breakthroughs of scRNA-seq and ST in plants, none have comprehensively addressed the use of these technologies to study plant responses to environmental stress at the cellular level. This review provides an in-depth analysis of the development, advantages, and limitations of single-cell and spatial transcriptomics, highlighting their critical role in unraveling plant strategies for coping with biotic and abiotic stresses. We also explore the challenges and future prospects of integrating scRNA-seq and ST in plant research. Understanding cell-specific responses and the complex interactions between cellular entities within the plant under stress is essential for advancing our knowledge of plant biology.