Salmonid fishes are a focal point of conservation physiology due to their high value to humans and ecosystems, and their susceptibility to decline from climate change. A significant challenge in conserving these fishes is that populations of the same species can be locally adapted to vastly different habitats within their wild ranges and can therefore have unique tolerance or vulnerability to environmental stressors within those habitats. Within the state of Oregon, USA, summer steelhead () inhabit both cool, coastal waters most typically associated with Pacific salmonids and arid, inland environments where temperatures are more extreme. Here, we utilized streamside physiological experiments paired with habitat temperature monitoring to assess the thermal tolerance and vulnerability of four populations of summer steelhead from distinct thermal habitats. All populations had unique responses of critical thermal maximum, aerobic scope and exercise recovery to temperature. Despite populations from warm habitats exhibiting higher thermal tolerance than populations from cooler habitats, summer steelhead from warm habitats appear to be more vulnerable to the physiological consequences of warming based on the extreme temperatures they already experience during the summer. These results demonstrate an example of thermal physiology varying between populations within the same portion of their latitudinal range and highlight the need for habitat-specific conservation strategies for this species.