Endotoxin, a component of the outer membrane of Gram-negative bacteria, has been extensively studied as a stimulator of the innate immune response. However, the temporal aspects and exposure-response relationship of endotoxin and resulting cytokine induction. This in vitro study aimed to investigate the factors influencing the interleukin-6 (IL-6) response to bacterial endotoxin stimulation. IL-6 is a pro-inflammatory cytokine that plays a crucial role in the immune response and inflammation.  In this study, we utilized an in vitro model to examine the factors influencing IL-6 production in response to bacterial endotoxin this experimental study was performed to find out the effect of healthy person on peripheral blood mononuclear cells(PBMCs) namely T-cells and natural killer cells(NK cells) function through challenging  their ability  for cytokines production in a comparative study by using of standardized inoculum of Bacterial endotoxins.