Hedyotis corymbosa (L.) Lam is a wild herb that is used in traditional Indian, Chinese, and African medicine. Light-emitting diode (LED) technology is paving the way to enhance crop production and inducing targeted photomorphogenic, biochemical, or physiological responses in plants. This study examines the efficiency of H. corymbosa (L.) Lam production under blue 450 nm and red 660 nm LED lights for overall plant growth, photosynthetic characteristics, and the contents of metabolite compounds. Our research showed that blue LED lights provided a positive effect on enhancing plant growth and overall biomass. In addition, blue LED lights are more effective in controlling the production of sucrose, starch, total phenolic compounds, and total flavonoid compared to red LED lights. However, blue and red LED lights played essential but different roles in photosynthetic characteristics. Our results showed the potential of colored LED light applications in improving farming methods and increasing metabolite production in herbs.