The biological synthesis of nanoparticles is an emerging field of study that seeks to synthesize nanoparticles using non-chemical mechanisms such as microorganisms, plants, and animal blood serum. Among these, plants have gained particular attention due to their ease of handling, availability, and ability to synthesize a wide range of nanoparticles. Therefore, the current study aimed to fabricate the silver nanoparticles (AgNPs) using Chinese medicinal plants (CMP) for their possible toxicity in common carp fish (Cyprinus carpio). For this purpose, CMP was dried, ground, and used as a bio-reductive agent. The fabricated AgNPs were characterized and a well dispersed AgNPs were obtained. Moreover, the C. carpio was exposed to the AgNPs for bioaccumulation and histological alterations. The obtained findings revealed that the AgNPs were mostly accumulated in the intestines followed by the gills, muscles, liver, and brain. The accumulated AgNPs caused histological alterations in gills and intestines at the highest concentration (0.08 mg/L). However, very less alterations were caused by the lowest concentration, especially in the intestine. In conclusion, further in-depth research is needed to determine the risks associated with the usage of nanoparticles to reveal their harmful impacts on fish and the aquatic environment. HIGHLIGHTS: The biological fabrication of AgNPs is considered eco-friendly. Chinese medicinal plants play a significant role in AgNPs synthesis. AgNPs have excellent antibacterial activity. AgNPs are bioaccumulated in various organs of fish.