The cardiac telocyte (TC) is a novel interstitial cell type with a unique ultrastructure and great potential in therapy. The present study examined its presence in the heart of chicken embryos ageing 7-15 days old (Hamburger-Hamilton [HH] stages 31-41) using transmission electron microscopy. TCs were identified across all stages in the atrial and ventricular myocardium, close to maturing cardiomyocytes, blood vessels and lymphatics. Early-stage TCs have immature features resembling mesenchymal cells. Late-stage TCs were distinct, possessing the cytoplasmic prolongations termed telopodes (Tps), which are very long and thin, usually 1-3 in number, and display a moniliform appearance and have an average thickness below 0.2 μm. TCs residing in the epicardium and endocardium were also detected. In the subepicardium near developing coronary vessels, they were localized in the cardiac stem cell niches, coexisting with cardiac stem cells and cardiomyocyte progenitors. Electron-dense structures and the release of extracellular vesicles were observed between embryonic TCs and surrounding structures, suggesting roles in intercellular communication, cardiomyocyte differentiation and maturation, angiogenesis, and stem cell nursing and guidance.