Simulation modeling of microbial proliferation had increased in recent years using Monod and advanced kinetic models. Accurate data is limited to advance studies in cell utilization for various applications due to complexity of the process. In this reasearch, behaviour of the Monod parameters at different substrate and biomass levels will be examined using predicted data obtained originally from empirical observations. Method involves deliberate variation of some variables to examine its effects on others starting from the basic Malthus equation. Results shows that, predicted data are more convinient and clearly presents the supposed dynamics of the process compared to ideal experimental results. By implications, improved procedures are necessary to allow for precise estimates of basic cell numbers and substrate concentration values.