The polyherbal formulation known as ELNA consists of Moringa oleifera, Crateva religiosa and Curcuma longa. It ethnomedicinal properties include; analgesic, inflammation, cardiac diseases, bacterial infection and immune suppressed diseases. The aim of this study was to evaluate the ex-vitro non-enzymatic anti-oxidant, biosafety, analgesic and anti-inflammatory effect of aqueous ELNA polyherbal extract. Ex-vitro anti-oxidant study was investigated in ELNA extract using standard radical scavenging protocol. Biosafety effect of ELNA extract was understudied with the aid of Locke method. Acetic acid-induced peripheral pain, hot plate-induced central pain and egg albumin-induced inflammatory models were investigated. Results from this study exhibited the scavenging effect of ELNA extract against 1, 1-diphenyl-2-picryhydroxyl with significant increase in concentration dependent manner. Acute toxicity study of the aqueous extract of ELNA elicited less or no toxic effect, with no mortality and side effect above LD50>5000 mg/kg body weight. Aqueous ELNA extract displayed no damaging effect of the sub-chronic toxicity study administered for 28 days, exhibited no significant difference (p= 0.05) in the organ and body weight. No significant different in the haematological parameters excluding 400 mg/kg with slight significant (p<0.05) increase in platelet count.  Graded doses of the extract at 400, 800 and 1200 mg/kg body weight exhibited significant (p<0.05) reduction in central and peripheral pain. Significant reduction in paw edema volume. Specifically at higher doses, the extract showed an effective activities against the selected study. In conclusion, this study validated the ethnomedicianl evidence of ELNA aqueous extract of the formulation.