Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme
Abstract
The glpK gene, which codes for Escherichia coli K-12 glycerol kinase (EC 2.1.7.30, ATP:glycerol 3-phosphotransferase), has been cloned into the HindIII site of pBR322. The gene was contained in a 2.8-kilobase DNA fragment which was obtained from a lambda transducing bacteriophage, lambda dglpK100 (C …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
amino acid sequence
P.H.S.
Molecular*
Genes
Base Sequence
Molecular Sequence Data
bacterial*
genes*
cloning
alleles
escherichia coli / genetics*
j r johnson
pmid:2826434
d w pettigrew
d p ma
escherichia coli / enzymology
regulator
glycerol kinase / genetics*
phosphotransferases / genetics*
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274756
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