Genetic incorporation of histidine derivatives using an engineered pyrrolysyl-tRNA synthetase
Abstract
A polyspecific amber suppressor aminoacyl-tRNA synthetase/tRNA pair was evolved that genetically encodes a series of histidine analogues in both Escherichia coli and mammalian cells. In combination with tRNACUA(Pyl), a pyrrolysyl-tRNA synthetase mutant was able to site-specifically incorporate 3-met …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
Molecular
pubmed abstract
nih
national institutes of health
national library of medicine
models
research support
N.I.H.
Extramural
mutation
letter
luminescent proteins / genetics
alanine / analogs & derivatives
protein engineering / methods*
escherichia coli / genetics*
mutant proteins / genetics
pmid:24506189
pmc4033645
doi:10.1021/cb500032c
han xiao
francis b peters
peter g schultz
alanine / genetics
amino acyl-trna synthetases / genetics*
histidine / analogs & derivatives*
histidine / genetics*
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274320
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