Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes

Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes

Betzl D;Ludwig W;Schleifer KH;;
Applied and environmental microbiology 1990 Vol. 56 pp. -
212
d1990appliedidentification

Abstract

Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizatio …

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