Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes
Betzl D;Ludwig W;Schleifer KH;;
Applied and environmental microbiology1990Vol. 56pp. -
212
d1990appliedidentification
Abstract
Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizatio …