Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions
Abstract
Atypical pathogens such as Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae are an important cause of community-acquired pneumonia. The available detection methods (culture and serology) either lack sensitivity or give only a retrospective diagnosis. In order to improve their d …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
Retrospective Studies
DNA
sensitivity and specificity
Bacterial / analysis
Polymerase Chain Reaction / methods*
biotechnology
pneumonia
bacterial / diagnosis*
mycoplasma pneumoniae / genetics
respiratory system / microbiology
mycoplasma / diagnosis
pmid:12614979
doi:10.1016/s0732-8893(02)00484-4
martine welti
katia jaton
jacques bille
chlamydophila infections / diagnosis
chlamydophila pneumoniae / genetics
chlamydophila pneumoniae / isolation & purification*
legionella pneumophila / genetics
legionella pneumophila / isolation & purification*
legionnaires' disease / diagnosis
mycoplasma pneumoniae / isolation & purification*
respiratory system / metabolism
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273818
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