Application of a fluorometric assay for characterization of the catalytic competency of a domain III fragment of Pseudomonas aeruginosa exotoxin A
Abstract
Pseudomonas aeruginosa exotoxin A (ETA) is a member of the family of bacterial ADP-ribosylating toxins that use NAD(+) as the ADP-ribose donor. The reaction catalyzed by ETA involves the nucleophilic attack of the diphthamide residue on the anomeric carbon of the nicotinamide ribose forming a new gl …
Keywords
catalysis
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
pubmed abstract
nih
national institutes of health
national library of medicine
reproducibility of results
research support
non-u.s. gov't
Temperature
drug combinations
osmolar concentration
protein structure
tertiary
peptide fragments / analysis
exotoxins / chemistry
virulence factors*
nad / metabolism
pmid:11319814
doi:10.1006/abio.2001.5052
s armstrong
a r merrill
adp ribose transferases*
adenosine diphosphate / metabolism
bacterial toxins*
exotoxins / analysis*
fluorometry / methods*
oils
peptide fragments / chemistry
phenols
poly(adp-ribose) polymerases / analysis
pseudomonas aeruginosa / chemistry
pseudomonas aeruginosa / enzymology*
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273544
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