We report a facile one step in-situ synthesis of amino-functionalized graphene dots. These quantum dots were employed for the detection of glucose in both standard aqueous solutions and commercially available fruit juice to assess its practicability. The characterization of the quantum dots revealed that they were decorated with amine functionality. Additionally, the interaction between glucose and amine functionalized graphene quantum dots gave enhancement in the UV-vis absorption and photoluminescence (PL) due to aggregation of quantum dots via glucose link. Therefore, the quantum dots were able to detect the concentration of glucose in solution exhibiting linearity from 0.1 to 10 mM and 50-500 mM with a sensitivity transition from 10 mM to 50 mM. The limit of detection for the determination of glucose was found to be 10 μM. This determination was agreed from both UV-Vis absorption and PL spectroscopy. However, the PL emission method of determination was most suited with its very high accuracy of 98.04 ± 1.96% and 97.33 ± 2.67% for the linear range of glucose concentration within 0.1-10 mM and 50-500 mM, respectively. The PL enhancement was highly selective towards glucose in mixture of other form of sugars making it suitable for determining glucose in food samples.