A simple method for the determination of reduction potentials in heme proteins
Abstract
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that t …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
spectrum analysis
oxidation-reduction
heme / chemistry*
pmid:24440354
pmc3999514
doi:10.1016/j.febslet.2013.12.030
igor efimov
gary parkin
emma l raven
basic helix-loop-helix transcription factors / chemistry*
clock proteins / chemistry*
fluorescent dyes / chemistry
hemeproteins / chemistry*
nerve tissue proteins / chemistry*
oxazines / chemistry
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270692
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