Senescence bypass screen identifies TBX2, which represses Cdkn2a (p19(ARF)) and is amplified in a subset of human breast cancers

Senescence bypass screen identifies TBX2, which represses Cdkn2a (p19(ARF)) and is amplified in a subset of human breast cancers

Jacobs JJ;Keblusek P;Robanus-Maandag E;Kristel P;Lingbeek M;Nederlof PM;van Welsem T;van de Vijver MJ;Koh EY;Daley GQ;van Lohuizen M;;
nature genetics 2000 Vol. 26 pp. -
347
jj2000naturesenescence

Abstract

To identify new immortalizing genes with potential roles in tumorigenesis, we performed a genetic screen aimed to bypass the rapid and tight senescence arrest of primary fibroblasts deficient for the oncogene Bmi1. We identified the T-box member TBX2 as a potent immortalizing gene that acts by downr …

Keywords

National Center for Biotechnology Information NCBI NLM MEDLINE genetic Mice gene expression regulation animals humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't u.s. gov't female P.H.S. Cells Cultured Genes neoplastic* human Fibroblasts / metabolism Promoter Regions protein biosynthesis* carrier proteins / genetics chromosomes transfection proteins / genetics tumor cells cell transformation oncogenes fibroblasts / cytology breast neoplasms / genetics* cell cycle proteins / genetics nuclear proteins / genetics dna-binding proteins* gene deletion Tumor Suppressor Protein p14ARF cell cycle proteins / physiology* neoplasm proteins / genetics neoplastic / genetics proto-oncogene proteins / genetics j j jacobs m van lohuizen polycomb repressive complex 1 adenocarcinoma / metabolism transcription factors / antagonists & inhibitors repressor proteins / genetics pmid:11062467 doi:10.1038/81583 p keblusek adenocarcinoma / genetics* breast neoplasms / metabolism cos cells cell cycle proteins / isolation & purification cellular senescence / genetics* pair 17 / genetics* cyclin-dependent kinase inhibitor p16 e2f transcription factors e2f1 transcription factor gene amplification* brca1 neoplasm proteins / biosynthesis neoplasm proteins / isolation & purification neoplasm proteins / physiology* neoplastic syndromes hereditary / genetics proto-oncogene proteins c-myc / antagonists & inhibitors proto-oncogene proteins p21(ras) / antagonists & inhibitors repressor proteins / isolation & purification repressor proteins / physiology* retinoblastoma-binding protein 1 t-box domain proteins / genetics t-box domain proteins / isolation & purification t-box domain proteins / physiology* transcription factor dp1

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