Robust production and passaging of infectious HPV in squamous epithelium of primary human keratinocytes
Hsu-Kun Wang, Aaron A. Duffy, Thomas R. Broker, Louise T. Chow;Hsu-Kun Wang;Aaron A. Duffy;Thomas R. Broker;Louise T. Chow;
genes & development2009Vol. 23pp. 181-
154
chow2009genesrobust
Abstract
Using Cre-loxP-mediated recombination, we established a highly efficient and reproducible system that generates autonomous HPV-18 genomes in primary human keratinocytes (PHKs), the organotypic raft cultures of which recapitulated a robust productive program. ...