Preparation and characterization of cell-free protein synthesis systems from oocytes and eggs of Xenopus laevis
Abstract
During the maturation of the oocytes of the frog Xenopus laevis, the rate of protein synthesis shows a twofold increase. Studies of the mechanisms involved in this stimulation have been seriously limited by the lack of an active cell-free translation system. We have now prepared such systems from oo …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
animals
pubmed abstract
nih
national institutes of health
national library of medicine
female
messenger / metabolism
kinetics
protein biosynthesis*
centrifugation
cell-free system
oocytes / drug effects
progesterone / pharmacology
pmid:2627883
t d patrick
c e lewer
v m pain
oocytes / growth & development
oocytes / metabolism*
ovum / metabolism*
transfer
amino acyl / metabolism
met*
xenopus laevis
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269741
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