As a common form of light detection and ranging (LiDAR) in forestry applications, the canopy height model (CHM) provides the elevation distribution of aboveground vegetation. A CHM is traditionally generated by interpolating all the first LiDAR echoes. However, the first echo cannot accurately represent the canopy surface, and the resulting large amount of noise (data pits) also reduce the CHM quality. Although previous studies concentrate on many pit-filling methods, the applicability of these methods in high-resolution unmanned aerial vehicle laser scanning (UAVLS)-derived CHMs has not been revealed. This study selected eight widely used, recently developed, representative pit-filling methods, namely first-echo interpolation, smooth filtering (mean, medium and Gaussian), highest point interpolation, pit-free algorithm, spike-free algorithm and graph-based progressive morphological filtering (GPMF). A comprehensive evaluation framework was implemented, including a quantitative evaluation using simulation data and an additional application evaluation using UAVLS data. The results indicated that the spike-free algorithm and GPMF had excellent visual performances and were closest to the real canopy surface (root mean square error (RMSE) of simulated data were 0.1578 m and 0.1093 m, respectively; RMSE of UAVLS data were 0.3179 m and 0.4379 m, respectively). Compared with the first-echo method, the accuracies of the spike-free algorithm and GPMF improved by approximately 23% and 22%, respectively. The pit-free algorithm and highest point interpolation method also have advantages in high-resolution CHM generation. The global smooth filter method based on the first-echo CHM reduced the average canopy height by approximately 7.73%. Coniferous forests require more pit-filling than broad-leaved forests and mixed forests. Although the results of individual tree applications indicated that there was no significant difference between these methods except the median filter method, pit-filling is still of great significance for generating high-resolution CHMs. This study provides guidance for using high-resolution UAVLS in forestry applications.