Isolation and characterization of endoplasmic reticulum and Golgi apparatus from hepatocyte nodules in male wistar rats
Abstract
A method is described for the isolation of endoplasmic reticulum and Golgi apparatus from hyperplastic liver nodules produced by discontinuous feeding of 2-acetylaminofluorene to male Wistar rats. The procedure involves three centrifugation steps and permits the separation of these cell components a …
Keywords
diet
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
animals
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
male
Rats
liver / drug effects
proteins / analysis
rna / analysis
inbred strains
liver / enzymology*
pmid:6189597
l c eriksson
u b torndal
g n andersson
2-acetylaminofluorene / toxicity
cell fractionation / methods*
centrifugation / methods
endoplasmic reticulum / enzymology
endoplasmic reticulum / ultrastructure
golgi apparatus / enzymology
golgi apparatus / ultrastructure
hyperplasia / chemically induced
lipids / analysis
liver / pathology*
microsomes
liver / ultrastructure
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269254
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