BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
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BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

Zabolocki, Michael;McCormack, Kasandra;Hurk, Mark van den;Milky, Bridget;Shoubridge, Andrew P.;Adams, Robert;Tran, Jenne;Mahadevan-Jansen, Anita;Reineck, Philipp;Thomas, Jacob;Hutchinson, Mark R.;Mak, Carmen K. H.;Añonuevo, Adam;Chew, Leon H.;Hirst, Adam J.;Lee, Vivian M.;Knock, Erin;Bardy, Cedric;
Nature communications 2020 Vol. 11 pp. 1-19
94
zabolocki2020brainphysnature

Abstract

Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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chemistry Biology (General) Chemical technology Engineering (General). Civil engineering (General) Technology Computer applications to medicine. Medical informatics Science (General) Science physics

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https://doi.org/10.1038/s41467-020-19275-x

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