image processing National Center for Biotechnology Information NCBI NLM MEDLINE Mice animals pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't N.I.H. Extramural Proto-Oncogene Proteins c-akt / metabolism Fatty Acids in situ hybridization organ culture techniques immunohistochemistry computer-assisted green fluorescent proteins / genetics insulin / metabolism leptin / metabolism signal transduction / physiology* neurons / metabolism* phosphatidylinositol 3-kinases / metabolism pmid:19074037 pmc2615536 doi:10.1523/jneurosci.4023-08.2008 makoto fukuda juli e jones joel k elmquist nonesterified / metabolism forkhead box protein o1 forkhead transcription factors / genetics forkhead transcription factors / metabolism* gene knock-in techniques green fluorescent proteins / metabolism* hypothalamus / metabolism