In this paper, Turbulent mixed convective heat transfer of water and Al2O3 nanofluid has been numerically studied in a horizontal tube under non-uniform heat flux on the upper wall and insulation in the lower wall using mixture model. For the discretization of governing equations, the second-order upstream difference scheme and finite volume method were used. The coupling of pressure and velocity was established by using SIMPLEC algorithm. The calculated results demonstrated that the convective heat transfer coefficient of nanofluid is higher than of the base fluid and by increasing the nanoparticles volume fraction, the convective heat transfer coefficient and shear stress on the wall increase. On the other hand, with increasing the Grashof number, the shear stress and convective heat transfer coefficient decrease.