The interaction of radiation with cells and tissues has a random nature. Therefore, understanding the random nature of cell killing that is determined by Poisson distribution statistics is an essential point in education of radiation biology. RadBioStat is a newly developed educational MATLAB-based software designed for computer-assisted learning of the target theory in radiation biology. Although its potential applications is developing rapidly, currently RadBioStat software can be a useful tool in computerassisted education of radiobiological models such as single target single hit, multiple target single hit and multiple target multiple hit. Scholars’ feedback is valuable to the producers of this software and help them continuously improve this product, add new features and increase its desirability and functionality.