Approved strategy of sustainable development of the Czech Republic is based on three pillars of defensibility, namely social, economic and environmental. The paper is focused on the third pillar that includes field of natural resources. It is solved in municipalities of chosen micro-regions in the Czech Republic compared with level in particular regions. In term of chosen indicators it is concerned with the coefficient analysis of ecological stability of landscape.Furthermore in the paper there are analysed chosen problems in the sphere of environment protection in particular districts where chosen micro-regions are situated. Analysis of environment protection is realized in years 2002–2008. Protection of environment is evaluated by indicators such as e.g. environmental assets and non-investment expenditures and emissions.