development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations

development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations

;Asad Raza;Abdul Subhan Ijaz;Shabbir Ahmad
greece and rome 2009 Vol. 32 pp. 1180-1183
146
raza2009qumicadevelopment

Abstract

A simple, fast and sensitive spectrophotometric method for the determination of cefaclor in pharmaceutical raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn's purple) that resulted from the reaction was stabilized and measured at 560 nm. Beer's law is obeyed in the concentration range of 4-80 µg mL-1 with molar absorptivity of 1.42 × 10(5) L mole-1 cm-1. All variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.

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0x95644003c57E6F55A65596E3D9Eac6813e3566dA
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259553
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10.1590/S0100-40422009000500018
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