We study the process pp→QV+X (where Q=t,b and V=g,γ, and Z) through the anomalous interactions of the new heavy quarks at the LHC. Considering the present limits on the masses and mixings, the signatures of the heavy quark anomalous interactions are discussed and analysed at the LHC for the center of mass energy of 13 TeV. An important sensitivity to anomalous couplings κgt′/Λ=0.10 TeV−1, κγt′/Λ=0.14 TeV−1, κZt′/Λ=0.19 TeV−1 and κgb′/Λ=0.15 TeV−1, κZb′/Λ=0.19 TeV−1, κγb′/Λ=0.30 TeV−1 for the mass of 750 GeV of the new heavy quarks t′ and b′ can be reached for an integrated luminosity of Lint=100 fb−1.