This paper outlines a study undertaken to determine if the electrical performance of Nickel Cobalt Aluminum Oxide (NCA) 3.1 Ah 18650 battery cells can be degraded by road induced vibration typical of an electric vehicle (EV) application. This study investigates if a particular cell orientation within the battery assembly can result in different levels of cell degradation. The 18650 cells were evaluated in accordance with Society of Automotive Engineers (SAE) J2380 standard. This vibration test is synthesized to represent 100,000 miles of North American customer operation at the 90th percentile. This study identified that both the electrical performance and the mechanical properties of the NCA lithium-ion cells were relatively unaffected when exposed to vibration energy that is commensurate with a typical vehicle life. Minor changes observed in the cell’s electrical characteristics were deemed not to be statistically significant and more likely attributable to laboratory conditions during cell testing and storage. The same conclusion was found, irrespective of cell orientation during the test.