Vortex tube is a simple device without a moving part which is capable of separating hot and cold gas streams from a higher pressure inlet gas stream. The mechanism of energy separation has been investigated by several scientists and second law approach has emerged as an important tool for optimizing the vortex tube performance. Here, a thermodynamic model has been used to investigate vortex tube energy separation. Further, a method has been proposed for optimizing the vortex tube based on the rate of entropy generation obtained from experiments. Also, an experimental study has been carried out to investigate the effects of the hot tube length and cold orifice diameter on entropy generation within a vortex tube with natural gas as working fluid. A comparison has been made between air and natural gas as working fluids. The results show that the longest tube generates lowest entropy for NG. For air, it is middle tube which generates lowest entropy. Integration of entropy generation for all available cold mass fractions unveiled that an optimized value for hot tube length and cold orifice diameter is exist.