Isolation, identification and grouping of staphylococcal enterotoxins in milk is of great importance, since these toxins are considered as a major potential source of humans illness. In this study, 75 staphylococcus aureus strains isolated from buffalo milk were assessed. For this, extracted DNA was analysed by multiplex-PCR for the presence and diversity of genes encoding the common classical staphylococcal enterotoxins. Results revealed that one of the isolates haboured both sec and seb genes; meanwhile, three other isolates contained merely sec gene. Moreover, the gene encoding the production of enterotoxin-A was not detected in any of the isolates. It was concluded that the diversity of genes encoding the classical enterotoxins of S. aureus isolated from Tabriz area was low.