A new clover slot antenna operating at 2.45 GHz Industrial, Scientific, and Medical (ISM) band for biomedical applications is presented and experimentally verified. By putting a single feed and truncating clover slots with extra perturbation, good performance of polarization can be achieved. Also, the miniaturized size of the proposed antenna is 14 × 12 × 0.8 mm3 by utilizing the clover shaped slots. A broader bandwidth of 2.5 GHz is obtained for reflection coefficient less than −10 dB. In addition, the radiation pattern of proposed antenna exhibits the maximum gain of −6 dBi.