This study presents a list of anurans in the northern region of the Tocantins River Basin (states of Tocantins and Maranhão), with information regarding seasonality, habitat, and microhabitat. We recorded 38 species in 15 genera and seven families. Most species (47 %) occurred exclusively in open areas and only 11 % of the species were found in forest habitats. Around 82 % of species were observed in ponds and only 7 % occurred in streams. Regarding seasonality, 54 % were observed both in the rainy and dry seasons. The northern area of the Cerrado has being rapidly converted in monocultures and information on its anuran fauna is limited. Additional information on its biodiversity is needed in order to provide the framework for conservation strategies.