This study is aimed at investigating the effect of using humic, bio and nano fertilizers and levels of nitrogen fertilizers in flower yield of saffron (Crocus sativus L.) at the University of Torbat Heydarieh research farm located in Zaveh in 2014-2015. Treatments consisted of three levels of nitrogen application and use of fertilizer sources as the main factor, including Bioumik, Super Humic, combined Super Humic and Bioumik, Humi Ful, Nitrokara and no fertilizer as sub plots as split plot based on randomized complete block design with 18 treatments and three replications. Analysis of data showed that the effect of year and fertilizer sources on all traits measured was significant. The effect of nitrogen treatments was significant (at 1%) except on number of flowers, dry style, mean dry weight stigma, and mean dry weight. Fertilizer sources increased all measured traits significantly. Application of Biomic increased petals and sepals dry weight (736.34 g/m2) by 46.78% in comparison with the control (464.19 g/m2). The highest dry weight stigma (524.2 g/m2) was seen in Super Humic + Bioumik treatment compared to the control group (443.1 g/m2). Super Humic treatment increased dry weight stigma by 86.49% relative to control. It seems that the use of humic, bio and nano fertilizers has a good effect on saffron performance.