Dengue Fever (DF) is a disease caused by the dengue virus that transmitted by Aedes aegypti (Ae. Aegypti) and Ae. albopictus. Dengue fever is now one of the most important public health problems in Indonesia. Vector control using insecticides is the most important strategy to control the DF. Massal fogging and selective abatisation have implementedd intensively to control Ae. aegypti. However after its a long time implementation, mosquitoes resistance and transovarial transmission have been reported. The aim of the study was to evaluate the susceptibility status of Ae. aegypti to organophosphate and its ability to transovarial transmit degue virus in Kendari City, South East Sulawesi. This was a observational study using cross-sectional design conducted in high endemic areas (Kadia Village) and low endemic areas (Kambu Village). Susceptibility status of Ae. aegypti larvae from F1’s egg generation was evaluated by biochemic assay, whereas the transovarial transmission of dengue virus of adult female Ae. aegypti was evaluated by immunohistochemistry method using head squash preparation. The results showed that the Ae. aegypti larvae resistant was higher in high endemic areas (Kadia Village) (83.33%) than in low endemica areas (Kambu Village) (60.00%). In addition, transovarial transmission index (TTI) of Ae. aegypti dengue virus in the high endemic areas (26%) was significantly higher than in the low endemic areas (12%) (p<0.05). In conclusion, the Ae. aegypti larvae resitance to organophosphate as well as the TTI in high endemic areas is higher than in low endemic area in Kendari, Sout East Sulawesi.