The aim of this study was to determine the differences in milkability, milk and health traits in Holstein and Simmental dairy cattle due to parity. Following traits were analysed: milkability (duration of milking, MT; milk yield per milking, MYM; maximal, MMF and average milk flow, AMF), milk traits (daily milk yield, DMY; daily fat content, DFC; daily protein content, DPC; daily lactose content, DLC; urea, UREA) and health traits (logarithmic somatic cell count, SCClog and teatend hyperkeratosis, TEH). Significant (p<0.05; p<0.01; p<0.0001) differences between parity in MYM, DMY, MMF, DPC and DLC was determined. Also, significant (p<0.0001) difference in the teat-end hyperkeratosis score were found between cows in 1st and 2nd as well as between cows in 1st and ≥3rd parity. The cows in 1st and 2nd parity had similar SCClog, while significant (p<0.05) difference was found between SCClog in cows in 1st and cows in ≥3rd parity. The obtained results indicate that the parity significantly affect the milkability, milk and health traits. Since the hyperkeratosis level is highly correlated to the mastitis incidence risk, the regular teat-end scoring should be used in order to ensure quality machine milking and to minimize mastitis incidence risk.