Backgound and Objectives: Premature newborns are exposed to multiple risk factors for late-onset sepsis: the immaturity of defense systems, low birth weight and low gestational age, use of invasive devices. Purpose to assess the occurrence and etiology of late-onset sepsis (ICSLC) in premature infants with birth weight less than 1500 g (VLBW) admitted to the Neonatal Intensive Care Unit (NICU) of a philanthropic reference in Belo Horizonte – Minas Gerais. Methodology: Cross-sectional study. We evaluated data from epidemiological surveillance system (SACIH®) and tokens active search of Hospital Infection Control Committee (HICC) of the institution, for January 2010 to December 2012. Results: were recorded in 1850 Health Associated Infection (HAI), these 1481 were of bloodstream infection (BSI), representing 80% of HAIs. Of ICS, 559 (37.7%) were early-onset and 922 (62.3%) late-onset. The density incidence of infections was 30.7 / 1.000patients-day. Were registered LCBI 256 patients, totaling 355 infections. Of these patients, 16 had LCBI more of a microbiological agent. 99 patients (38.6%) had more than one LCBI. Birth-weight average 985g and gestational age (GA) average of 28 weeks. Most frequent agents were Coagulase-Negative Staphylococcus 49.9% (n = 117), Klebsiella sp 17% (n = 60), Candida sp 12.4% (n = 44). The lethality rate associated with LCBI in VLBW was 6.8% (n = 23). Conclusion: Despite the technological advances in fetal medicine and increase the survival of VLBW infants, the late onset sepsis presents itself at high rates and high incidence, which represents a major cause of morbidity and mortality for these newborns. KEYWORDS: Sepsis. Premature. Newborn. Infection control.