Plants have extraordinary developmental plasticity as they continuously form organs duringpostembryonic development. In addition they may regenerate organs upon in vitro hormonalinduction. Advances in the field of plant regeneration show that the first steps of de novoorganogenesis through in vitro culture in hormone containing media (via formation of aproliferating mass of cells or callus) require root postembryonic developmental programs as wellas regulators of auxin and cytokinin signaling pathways. We review how hormonal regulation isdelivered during lateral root initiation and callus formation. Implications in reprograming, cellfate and pluripotency acquisition are discussed. Finally, we analyze the function of cell-cycleregulators and connections with epigenetic regulation. Future work dissecting plantorganogenesis driven by both endogenous and exogenous cues (upon hormonal induction) mayreveal new paradigms of common regulation.